Bioconductor Developer Survey 2026 Now Open!

recountWorkflow

This is the released version of recountWorkflow; for the devel version, see recountWorkflow.

All versions 3.24 (devel), 3.23 (release), 3.22, 3.21, 3.20, 3.19, 3.18, 3.17, 3.16, 3.15, 3.14, 3.13, 3.12, 3.11, 3.10, 3.9, 3.8, 3.7

recount workflow: accessing over 70,000 human RNA-seq samples with Bioconductor


Bioconductor version: Release (3.23)

The recount2 resource is composed of over 70,000 uniformly processed human RNA-seq samples spanning TCGA and SRA, including GTEx. The processed data can be accessed via the recount2 website and the recount Bioconductor package. This workflow explains in detail how to use the recount package and how to integrate it with other Bioconductor packages for several analyses that can be carried out with the recount2 resource. In particular, we describe how the coverage count matrices were computed in recount2 as well as different ways of obtaining public metadata, which can facilitate downstream analyses. Step-by-step directions show how to do a gene level differential expression analysis, visualize base-level genome coverage data, and perform an analyses at multiple feature levels. This workflow thus provides further information to understand the data in recount2 and a compendium of R code to use the data.

Author: Leonardo Collado-Torres [aut, cre], Abhinav Nellore [ctb], Andrew E. Jaffe [ctb]

Maintainer: Leonardo Collado-Torres <lcolladotor at gmail.com>

Citation (from within R, enter citation("recountWorkflow")):

Leonardo Collado-Torres. recountWorkflow: recount workflow: accessing over 70,000 human RNA-seq samples with Bioconductor. doi:10.18129/B9.bioc.recountWorkflow, R package version 1.36.0, https://bioconductor.org/packages/recountWorkflow.

Generated from the package metadata; it may differ from the package's own citation.

Installation

To install this package, start R (version "4.6") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("recountWorkflow")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("recountWorkflow")
recount workflow: accessing over 70,000 human RNA-seq samples with Bioconductor HTML R Script
NEWSText

Details

biocViews ResourceQueryingWorkflow, Workflow
Version1.36.0
In Bioconductor sinceBioC 3.7 (R-3.5) (8.5 years)
License Artistic-2.0
Depends R (>= 3.6.0)
Imports recount, GenomicRanges, limma, edgeR, DESeq2, pheatmap, regionReport, clusterProfiler, org.Hs.eg.db, gplots, derfinder, GenomicState, bumphunter, derfinderPlot
System Requirements
URLhttps://github.com/LieberInstitute/recountWorkflow
Bug Reportshttps://support.bioconductor.org/t/recountWorkflow/
See More
Suggests BiocStyle, BiocWorkflowTools, knitr, magick, sessioninfo, rmarkdown
Linking To
Enhances
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report Build Report, r-universe

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package recountWorkflow_1.36.0.tar.gz
Windows Binary (x86_64)
macOS Binary (big-sur-x86_64)
macOS Binary (sonoma-arm64)
Source Repositorygit clone https://git.bioconductor.org/packages/recountWorkflow
Source Repository (Developer Access)git clone git@git.bioconductor.org:packages/recountWorkflow
Package Short Url https://bioconductor.org/packages/recountWorkflow/
Package Downloads ReportDownload Stats