1 Basics

1.1 Install chevreulPlot

R is an open-source statistical environment which can be easily modified to enhance its functionality via packages. chevreulPlot is a R package available via the Bioconductor repository for packages. R can be installed on any operating system from CRAN after which you can install chevreulPlot by using the following commands in your R session:

if (!requireNamespace("BiocManager", quietly = TRUE)) {
    install.packages("BiocManager")
}

BiocManager::install("chevreulPlot")

1.2 Required knowledge

The chevreulPlot package is designed for single-cell RNA sequencing data. The functions included within this package are derived from other packages that have implemented the infrastructure needed for RNA-seq data processing and analysis. Packages that have been instrumental in the development of chevreulPlot include, Biocpkg("SummarizedExperiment") and Biocpkg("scater").

1.3 Asking for help

R and Bioconductor have a steep learning curve so it is critical to learn where to ask for help. The Bioconductor support site is the main resource for getting help: remember to use the chevreulPlot tag and check the older posts.

2 Quick start to using chevreulPlot

The chevreulPlot package contains functions to preprocess, cluster, visualize, and perform other analyses on scRNA-seq data. It also contains a shiny app for easy visualization and analysis of scRNA data.

chvereul uses SingelCellExperiment (SCE) object type (from SingleCellExperiment) to store expression and other metadata from single-cell experiments.

This package features functions capable of:

  • Performing Clustering at a range of resolutions and Dimensional reduction of Raw Sequencing Data.
  • Visualizing scRNA data using different plotting functions.
  • Integration of multiple datasets for consistent analyses.
  • Cell cycle state regression and labeling.

library("chevreulPlot")

# Load the data
data("small_example_dataset")
sessionInfo()
#> R version 4.6.0 Patched (2026-05-01 r89994)
#> Platform: aarch64-apple-darwin23
#> Running under: macOS Tahoe 26.3.1
#> 
#> Matrix products: default
#> BLAS:   /Library/Frameworks/R.framework/Versions/4.6/Resources/lib/libRblas.0.dylib 
#> LAPACK: /Library/Frameworks/R.framework/Versions/4.6/Resources/lib/libRlapack.dylib;  LAPACK version 3.12.1
#> 
#> locale:
#> [1] C/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
#> 
#> time zone: America/New_York
#> tzcode source: internal
#> 
#> attached base packages:
#> [1] stats4    stats     graphics  grDevices utils     datasets  methods  
#> [8] base     
#> 
#> other attached packages:
#>  [1] chevreulPlot_1.5.0          chevreulProcess_1.5.0      
#>  [3] scater_1.41.1               ggplot2_4.0.3              
#>  [5] scuttle_1.23.1              SingleCellExperiment_1.35.1
#>  [7] SummarizedExperiment_1.43.0 Biobase_2.73.1             
#>  [9] GenomicRanges_1.65.0        Seqinfo_1.3.0              
#> [11] IRanges_2.47.1              S4Vectors_0.51.2           
#> [13] BiocGenerics_0.59.2         generics_0.1.4             
#> [15] MatrixGenerics_1.25.0       matrixStats_1.5.0          
#> [17] BiocStyle_2.41.0           
#> 
#> loaded via a namespace (and not attached):
#>   [1] RColorBrewer_1.1-3        jsonlite_2.0.0           
#>   [3] shape_1.4.6.1             magrittr_2.0.5           
#>   [5] ggbeeswarm_0.7.3          GenomicFeatures_1.65.0   
#>   [7] farver_2.1.2              rmarkdown_2.31           
#>   [9] GlobalOptions_0.1.4       fs_2.1.0                 
#>  [11] BiocIO_1.23.3             vctrs_0.7.3              
#>  [13] memoise_2.0.1             Rsamtools_2.29.0         
#>  [15] DelayedMatrixStats_1.35.0 RCurl_1.98-1.18          
#>  [17] forcats_1.0.1             htmltools_0.5.9          
#>  [19] S4Arrays_1.13.0           BiocBaseUtils_1.15.1     
#>  [21] curl_7.1.0                BiocNeighbors_2.7.2      
#>  [23] SparseArray_1.13.2        sass_0.4.10              
#>  [25] bslib_0.11.0              htmlwidgets_1.6.4        
#>  [27] plotly_4.12.0             cachem_1.1.0             
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#>  [31] igraph_2.3.1              iterators_1.0.14         
#>  [33] lifecycle_1.0.5           pkgconfig_2.0.3          
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#>  [37] R6_2.6.1                  fastmap_1.2.0            
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#>  [49] abind_1.4-8               compiler_4.6.0           
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#>  [55] BiocParallel_1.47.0       viridis_0.6.5            
#>  [57] DBI_1.3.0                 DelayedArray_0.39.2      
#>  [59] rjson_0.2.23              bluster_1.23.0           
#>  [61] tools_4.6.0               vipor_0.4.7              
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#>  [65] glue_1.8.1                restfulr_0.0.16          
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#>  [87] dplyr_1.2.1               lattice_0.22-9           
#>  [89] rtracklayer_1.73.0        bit_4.6.0                
#>  [91] tidyselect_1.2.1          ComplexHeatmap_2.29.0    
#>  [93] locfit_1.5-9.12           Biostrings_2.81.1        
#>  [95] knitr_1.51                gridExtra_2.3            
#>  [97] bookdown_0.46             ProtGenerics_1.45.0      
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#> [105] EnsDb.Hsapiens.v86_2.99.0 lazyeval_0.2.3           
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#> [109] codetools_0.2-20          cigarillo_1.3.0          
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#> [123] blob_1.3.0                AnnotationFilter_1.37.0  
#> [125] scran_1.41.0              sparseMatrixStats_1.25.0 
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